PROJECT LAB-MOLECULAR GENETICS

Multicellular animals contain a diverse array of cell types, yet start from a single cell. How do cells decide what kind of cell to be? In this lab course, we will use the tools of molecular biology and genetics to explore this fascinating question. We will use the nematode Caenorhabditis elegans, a powerful model organism used in hundreds of research labs. The course will be divided into three modules: C. elegans genetics, molecular cloning, and genetic screening. Laboratory techniques will include PCR, gel electrophoresis, restriction digest, ligation, transformation, RNAi, and C. elegans maintenance. Students will pursue original projects; emphasis will be placed on scientific thinking and scientific communication. SPS and TC students may register for this course, but they must first obtain the written permission of the instructor, by filling out a paper Registration Adjustment Form (Add/Drop form). Prerequisites: UN2005/UN2401 and UN2006/UN2402, or the equivalent at a different institution.